Mouse IgG1 Kappa Light Chain Isotype Control FITC

General information
 

Name:
Mouse IgG1 Kappa Light Chain Isotype Control FITC
Size:
100 tests
Catalog no:
61R-I170aFT
Price:
290 EUR
 

Additional extra details
 

  • Category

    Biological Reagents

    Antibody Subtype

    Isotype Controls

    Area of research

    Immunology

  • Type of Immunogen

    Raised in

    Mouse

    Cross Reactivity

  • Isotypes

    IgG1 kappa

    Clone

    M12637

    Form & Buffer

    Affinity purified and Provided in PBS, pH 7.2, with 0.2% BSA and 0.09% NaN3.

  • Storage

    Store at 4 deg C. DO NOT FREEZE. Keep away from light.

    Shipping conditions

    Blue Ice

    Tested for

    ICC; IF; IHC; IP; FC; WB

  • Usage Recommendations

    FC: 20 ul per 106 cells in 100 ul volume

    Assay Information

    This immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

    Tissue

    control

  • Description

    Isotype or positive controls by peptides, antibodies and deactivated samples.

    Properties

    This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

    Conjugation

    Anti-FITC Antibody

  • Group

    isotypes

    Test

    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

    Latin name

    Mus musculus