IgM Isotype Control Fc fusion protein FITC

General information
 

Name:
IgM Isotype Control Fc fusion protein FITC
Size:
50 µg
Catalog no:
35R-0009
Price:
204 EUR
 

Additional extra details
 

  • Category

    Biological Reagents

    Antibody Subtype

    Isotype Controls

    Area of research

    Immunology

  • Raised in

    Rat

    Isotypes

    IgM kappa

    Concentration

    200 ug/mL

  • Form & Buffer

    Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.

    Storage

    Store at 2-8 deg C. Do not freeze. Protect from light.

    Shipping conditions

    Blue Ice

  • Tested for

    FC

    Usage Recommendations

    20 ul (1 ug)/test

    Assay Information

    Each test size contains 0.5 ug. The pre-titrated test size contains 0.2% BSA.

  • Additional Information

    This antibody is offered in 2 formats.

    Tissue

    control

    Description

    Isotype or positive controls by peptides, antibodies and deactivated samples.Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.

  • Properties

    This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen,Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

    Conjugation

    Anti-FITC Antibody

    Additional isotype

    IgM

  • Group

    isotypes