IgG2a Isotype Control Fc fusion protein biotin

General information
 

Name:
IgG2a Isotype Control Fc fusion protein biotin
Size:
50 µg
Catalog no:
35R-0023
Price:
167 EUR
 

Additional extra details
 

  • Category

    Biological Reagents

    Antibody Subtype

    Isotype Controls

    Area of research

    Immunology

  • Raised in

    Rat

    Isotypes

    IgG2a kappa

    Concentration

    500 ug/ml

  • Form & Buffer

    Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.

    Storage

    Store at 2-8 deg C. Do not freeze. Protect from light.

    Shipping conditions

    Blue Ice

  • Tested for

    FC

    Usage Recommendations

    FC: ≤ 0.25 ug/test

    Assay Information

    A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest

  • Tissue

    control

    Properties

    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

    Conjugation

    Biotinylated

  • Description

    Isotype or positive controls by peptides, antibodies and deactivated samples.Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.

    Group

    isotypes