IgG1 Isotype Control Fc fusion protein

General information
 

Name:
IgG1 Isotype Control Fc fusion protein
Size:
50 µg
Catalog no:
35R-0026
Price:
167 EUR
 

Additional extra details
 

  • Category

    Biological Reagents

    Antibody Subtype

    Isotype Controls

    Area of research

    Immunology

  • Raised in

    Rat

    Isotypes

    IgG1 kappa

    Method of Purification

    IgG1 Isotype Control Fc fusion protein was purified by affinity chromatography.

  • Concentration

    500 ug/ml

    Form & Buffer

    Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.

    Storage

    Store at 2-8 deg C. Do not freeze. Protect from light.

  • Shipping conditions

    Blue Ice

    Tested for

    FC; IP; WB

    Usage Recommendations

    FC: ≤ 1 ug/test

  • Assay Information

    A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest

    Tissue

    control

    Description

    Isotype or positive controls by peptides, antibodies and deactivated samples.Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.

  • Properties

    Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

    Group

    isotypes