IgG Isotype Control antibody FITC

General information
 

Name:
IgG Isotype Control antibody FITC
Size:
50 µg
Catalog no:
61R-1136
Price:
204 EUR
 

Additional extra details
 

  • Category

    Biological Reagents

    Antibody Subtype

    Isotype Controls

    Area of research

    Immunology

  • Raised in

    Hamster

    Isotypes

    IgG

    Clone

    299Arm

  • Conjugate/Tag

    FITC

    Concentration

    500 ug/ml

    Form & Buffer

    Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.

  • Storage

    Store at 2-8 deg C. Do not freeze. Protect from light.

    Shipping conditions

    Blue Ice

    Tested for

    FC

  • Usage Recommendations

    FC: ≤ 1 ug/test

    Assay Information

    A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest

    Additional Information

    This antibody is offered in 2 formats.

  • Tissue

    control

    Properties

    If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

    Description

    Isotype or positive controls by peptides, antibodies and deactivated samples.

  • Conjugation

    Anti-FITC Antibody

    About

    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

    Additional isotype

    IgG

  • Group

    isotypes

    French translation

    anticorps