IgG Isotype Control antibody biotin

General information
 

Name:
IgG Isotype Control antibody biotin
Size:
50 µg
Catalog no:
61R-1586
Price:
204 EUR
 

Additional extra details
 

  • Category

    Biological Reagents

    Antibody Subtype

    Isotype Controls

    Area of research

    Immunology

  • Raised in

    Hamster

    Specificity

    Human

    Isotypes

    IgG

  • Clone

    299Arm

    Conjugate/Tag

    Biotin

    Concentration

    500 ug/ml

  • Form & Buffer

    Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.

    Storage

    Store at 2-8 deg C. Do not freeze. Protect from light.

    Shipping conditions

    Blue Ice

  • Tested for

    FC

    Usage Recommendations

    FC: ≤ 0.25 ug/test

    Assay Information

    A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest

  • Tissue

    control

    Properties

    If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

    Conjugation

    Biotinylated

  • Description

    Isotype or positive controls by peptides, antibodies and deactivated samples.

    About

    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

    Additional isotype

    IgG

  • Group

    isotypes

    French translation

    anticorps