Rat Kidney antibody Control Lysate

General information
 

Name:
Rat Kidney antibody Control Lysate
Size:
100uL
Catalog no:
X1277
Price:
104 EUR
 

Additional extra details
 

  • Category

    Other Reagents

    Long description

    Total cell Iysates are useful as both positive and negative controls in immunoblotting. A431, a human epidermoid carcinoma cell line, and the EGF-stimulated A431 Iysates are used as negative and positive controls, respectively, when studying the phosphorylation cascade initiated by ligand binding to receptor tyrosine kinases. Both A431 and normal human fibroblasts are valuable positive controls for antibodies to proteins expressed in these cells. The mouse fibroblast cell line 3T3 and its RSV-transformed counterpart serve as negative and positive controls, respectively, for antibodies to proteins that are phosphorylated as a result of transformation by RSV (Rous sarcoma virus). Madin-Darby bovine kidney (MDBK) cell Iysate displays proteins expressed in bovine kidney and PC12 (rat phaeochromocytoma) cell Iysate can be expected to mirror the protein expression of cells and tissues of neuroectodermal origin. Likewise, Jurkat cells express the proteins unique to human T-cells. Rat brain is a widely used positive control for those proteins that are exclusively expressed in brain. At right is an example of the use of positive and negative control Iysates in western blotting. _x000B__x000B_The blot of 3T3 cell Iysate (lane 1) and RSV-transformed 3T3 (lane 2) was probed with anti-phosphotyrosine peroxidase conjugate to reveal those proteins that are tyrosine-phosphorylated in response to transformation by Rous sarcoma virus.

    Antibody come from

    Hybridoma produced by the fusion of spelocytes from BALB/c mice immunized with partially purified rat brain G i1 protein and mouse myeloma Ag8 (NS1) cells.

  • Other description

    Provided as solution in phosphate buffered saline with 0.08% sodium azide.

    Clone

    not specified

    Antigen-antibody binding interaction

    Rat Kidney antibody Control Lysate

  • Antibody is raised in

    see techfile

    Antibody's reacts with

    see techfile

    Antibody's reacts with these species

    This antibody doesn't cross react with other species

  • Antibody's specificity

    No Data Available

    Application

    Western Blot

    Antibody's suited for

    This antibody can be used for Western blotting (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.

  • Storage

    -20ºC

    Relevant references

    1. Li X, et al. Pertussis toxin-sensitive G protein alpha-subunits: production of monoclonal antibodies and detection of differential increases on differentiation of PC12 and LA-N-5 cells. J. Neurochem. 1995, 64, 1107-1117.

    Protein number

    see ncbi

  • Warnings

    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

    Tissue

    control, kidney

    Properties

    If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

  • Description

    Isotype or positive controls by peptides, antibodies and deactivated samples.

    About

    Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

    Latin name

    Rattus norvegicus

  • French translation

    anticorps