Category

Other Reagents

Long description

NCC-4 (also known as HCC-4) is a CC chemokine. It is a 97 amino acid protein. It exhibits less than 30% sequence identity to other CC chemokines. NCC-4 chemoattracts human monocytes and THP-1 cells. However it does not chemoattract resting lymphocytes or neutrophils. It has been found to supress proliferation of myeloid progenitor cells and induces calcium flux in THP-1 cells. This calcium flux can be desensitized by exposure to RANTES which suggests that NCC-4 and RANTES share the same receptor in THP-1 cells.

Antibody come from

Hybridoma produced by the fusion of splenocytes from mice immunized with protein preparation from a homogenate of a human mammary tumor and mouse myeloma cells.

Other description

Provided as solution in phosphate buffered saline with 0.08% sodium azide.

Clone

not specified

Antigen-antibody binding interaction

NCC4 - Control Lysate

Antibody is raised in

see techfile

Antibody's reacts with

see techfile

Antibody's reacts with these species

This antibody doesn't cross react with other species

Antibody's specificity

No Data Available

Application

Activity Assay

Antibody's suited for

Detects human tenascin protein by Western blot at 1-5 µg/ml. Can also be used for immunohistochemistry on frozen and paraffin-embedded tissue sections and for immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.

Storage

-20ºC

Relevant references

1. Verstraeten, A.A. et al. Tenascin expression in basal cell carcinoma. Br. J. Dermatol. 1992, 127, 571-574_x000B__x000B_2. Le Poole, I.C., et al. Tenascin is overexpressed in vitiligo lesional skin and inhibits melanocyte adhesion. Br. J. Dermatol. 1997, 137, 171-178_x000B__x000B_3. Rulo, H.F., et al. Normal human skin demonstrates marked site-variation of tenascin expression, not correlated to epidermal proliferation (Ki-67 binding). J. Dermatol. Sci. 1993, 5, 54-57

Protein number

see ncbi

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Tissue

control

Description

Isotype or positive controls by peptides, antibodies and deactivated samples.