Category

Three-Color Reagents (FITC/RPE/Cy5RPE)

Long description

Negativecontrol for immunofluorescence staining with Exalpha Biological's 3-ColorTM FITC, RPE and RPE-Cy-5 of mouse monoclonal antibodies. Assessment of non-specific binding of mouse monoclonal antibodies to human cell surface antigens.

Antibody come from

n/a

Other description

Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Purified from mouse ascetic fluid by affinity chromatography.

Clone

not specified

Antigen-antibody binding interaction

Mouse IgG1 antibody conjugated to FITC / IgG1 RPE / IgG1 RPE-Cy5 - Negative Control Antibody

Antibody is raised in

Mouse

Antibody's reacts with

Human

Antibody's reacts with these species

This antibody doesn't cross react with other species

Antibody's specificity

No Data Available

Research interest

CD Marker

Application

Flow Cytometry

Antibody's suited for

PBMC: Add 10 µl of 3-ColorTM antibody reagent/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼ C. Wash twice with PBS. Add 10 µl of Exalpha Biological's pre-titered 3-ColorTM and incubate 15 minutes at 2¼ to 8¼ C. Wash twice with PBS or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of Exalpha Biological's 3-ColorTM antibody reagent MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20¼ C. Lyse the whole blood. Wash twice with PBS or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Storage

4ºC

Relevant references

1. Protection of Laboratory Workers from occupationally acquired infections. Second edition; approved guideline (2001). Villanova PA: National Committee for Clinical Laboratory Standards; Document M29-A2._x000B_2. Procedures for the collection of diagnostic blood specimens by venipuncture- approved standard; Fifth edition (2003). Wayne PA: National Committee for Clinical Laboratory Standards; Document H3-A5._x000B_3. Clinical applications of flow cytometry: Quality assurance and immunophenotyping of lymphocytes; approved guideline (1998). Wayne PA: National Committee for Clinical Laboratory Standards; Document H42-A._x000B__x000B_Cy-5+ Portions of this product is manufactured under license from Carnegie Mellon University, U.S. Patent Number 5,268,486.

Protein number

see ncbi

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Properties

If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.

Description

Isotype or positive controls by peptides, antibodies and deactivated samples.Negative control peptides or isotypes available of the different species.

Conjugation

Cy5.5,Anti-FITC Antibody

Additional isotype

IgG1

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus

French translation

anticorps