IgG1 Platelet antibody conjugated to PE - Negative Control

General information
 

Name:
IgG1 Platelet antibody conjugated to PE - Negative Control
Size:
100 Tests
Catalog no:
0PPE
Price:
195 EUR
 

Additional extra details
 

  • Category

    Negative Control

    Long description

    Negative control for Immunofluorescence staining with PE of mouse monoclonal antibodies. Assessment of non-specific binding of mouse monoclonal antibodies to human platelets.

    Antibody come from

    n/a

  • Other description

    Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography

    Clone

    ZX3

    Antigen-antibody binding interaction

    Mouse IgG1 Platelet antibody conjugated to PE - Negative Control

  • Antibody is raised in

    Mouse

    Antibody's reacts with

    see techfile

    Antibody's reacts with these species

    This antibody doesn't cross react with other species

  • Antibody's specificity

    No Data Available

    Application

    Flow Cytometry

    Antibody's suited for

    Collect blood aseptically by venipuncture into an ACD or EDTA blood collection tube. Important: Within 5 minutes of blood collection, fix blood sample by placing 100_l of blood in test tube containing 1 ml of cold (4¼ C) 1X PBS with 1% paraformaldehyde. Mix by vortexing. Centrifuge the fixed blood at 1200 x g for 5 minutes at room temperature (RT) 20¼C. Aspirate the supernatant, leave pellet. Prior to staining, wash the fixed blood pellet 2X with 1 ml 1X PBS + 0.1% Azide at RT. Centrifuge the fixed blood at 1200 x g for 5 minutes at room temperature (RT) 20¼C. Aspirate the supernatant, leave pellet. Resuspend the pellet in 1 ml of 1X PBS at RT. To a clean labeled test tube add 10 µl of Exalpha monoclonal antibody. Carefully add 50 µl of the fixed blood suspension to the bottom of the test tube. Vortex and incubate at room temperature for at least 15 minutes and analyzed within 3 hours. Please note: Using this procedure will yield twenty samples for platelet analysis. Select logarithmic (Log) amplification for both Forward (FSC) and Side (SSC) scatters, while collecting data for platelets. See instrument manufacturerÕs instructions for immunofluorescence analysis with a flow cytometer or microscope

  • Storage

    4ºC

    Relevant references

    no information yet

    Protein number

    see ncbi

  • Warnings

    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

    Properties

    If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

    Description

    Isotype or positive controls by peptides, antibodies and deactivated samples.Negative control peptides or isotypes available of the different species.Platelets, also called thrombocytes or cloth cells in blood and are needed to stop bleeding by clumping and clotting the blood the vessels when the an injury occurs. Teh bone marrow will produce the platelets that have no nucleus. Platelates are unique to mammals, the are curved shaped 1900nm to 3100 nm large nucleus free clothing structures.

  • Additional isotype

    IgG1

    French translation

    anticorps