Mouse IgG2a, Clone: F10-89-4, Mouse Monoclonal antibody-Human CD45; Negative Control for Rat Tissues ONLY

General information
 

Name:
Mouse IgG2a, Clone: F10-89-4, Mouse Monoclonal antibody-Human CD45; Negative Control for Rat Tissues ONLY
Size:
100 tests
Catalog no:
OBT0293
Price:
5 EUR
 

Additional extra details
 

  • Alternative name 1

    Mouse IgG2a, Clone: F10-89-4, Mab anti-Human CD45; Negative Control for Rat Tissues ONLY

    Alternative name 2

    Mouse IgG2a, Clone: F10-89-4, Mab antibody-Human CD45; Negative Control for Rat Tissues ONLY

    Alternative name 3

    Mus musculus IgG2a, Clone: F10-89-4, Mab antibody-Homo sapiens CD45; Negative Control for Rattus norvegicus Tissues ONLY

  • Alternative name 4

    anti- IgG2a Clone: F10-89-4 CD45 Negative Control for Tissues ONLY

    Alternative name 5

    IgG2a Clone: F10-89-4 CD45 Negative Control for Tissues ONLY

    Clonality

    Monoclonal Antibody

  • Description

    This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.

    Storage and handling

    Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.

    Advisory

    To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.

  • Tissue

    control

    Properties

    If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

    Additional description

    Isotype or positive controls by peptides, antibodies and deactivated samples.Negative control peptides or isotypes available of the different species.

  • About

    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

    Test

    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

    Latin name

    Mus musculus,Rattus norvegicus

  • French translation

    anticorps