Rat IgG2a, Negative Control for use in Human tissues (clone: LODNP16), FITC

General information
 

Name:
Rat IgG2a, Negative Control for use in Human tissues (clone: LODNP16), FITC
Size:
1 mg
Catalog no:
OBT4264F
Price:
5 EUR
 

Additional extra details
 

  • Alternative name 1

    Rat IgG2a, Negative Control for use in Human tissues (clone: LODNP16), FITC

    Alternative name 2

    Rat IgG2a, Negative Control for use in Human tissues (clone: LODNP16), FITC

    Alternative name 3

    Rattus norvegicus IgG2a, Negative Control for use in Human tissues (clone: LODNP16), FITC

  • Alternative name 4

    anti- IgG2a Negative Control for use in tissues clone: LODNP16

    Alternative name 5

    IgG2a Negative Control for use in tissues clone: LODNP16

    Clonality

    Monoclonal Antibody

  • Description

    This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.

    Storage and handling

    Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.

    Advisory

    To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.

  • Tissue

    control

    Additional description

    Isotype or positive controls by peptides, antibodies and deactivated samples.Negative control peptides or isotypes available of the different species.

    Properties

    This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

  • Conjugation

    Anti-FITC Antibody

    About

    Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

    Latin name

    Rattus norvegicus