Goat IgG (-ve control for flow cytometry) (isotype control)

General information
 

Name:
Goat IgG (-ve control for flow cytometry) (isotype control)
Size:
100 test
Catalog no:
20011-100
Price:
102 EUR
 

Additional extra details
 

  • Stock availability

    Available

    Category

    Isotype Control

    Antibody type

    N/A

  • Antibody host

    N/A

    Antibody conjugate

    N/A

    Technical datasheet

    Contact Gentaur to request the datasheet or ask our specialists for more information.

  • Notes

    The Goat IgG (-ve control for flow cytometry) (isotype control) is manufactured for Research Use Only or for diagnostics purposes.

    Tissue

    control

    Description

    Isotype or positive controls by peptides, antibodies and deactivated samples.FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors.

  • Test

    Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.

    Latin name

    Capra aegagrus hircus

    About

    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

  • Additional isotype

    IgG

    Properties

    Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

    Group

    isotypes