Detection on formaling fixed tissues
Nichirei histofine detection reagents, 2 steps are for eliminating background in immuno-histochemical flex and quanto staining of formalin-fixed and paraffin-embedded tissues. The ultra-view and vision of IHC tissue sections by DAB thermo dako staining uses a HRP horse red peroxidase ready-to-use high sensitive enhanced color reaction with a Hematoxilyn counterstaining. This double stain solution is flexible on autostainer with atlas primary antibodies due to the fab' fragment used in the secondairy atlas tissue staining. Stainings with dual link staining systems have similar results. Fisher, M. E. et al. Comparing 3D scientific tissue models.
Use on paraffin embedded tissues
Nicherei advises for IHC staining to use the Histofine ALK Control Slides
Isotype or positive controls by peptides, antibodies and deactivated samples.