Category

Antibody

Concentration

0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide

Form

Purified antibody

Conjugation

Biotin Conjugate

Clone

Polyclonal antibody

Recognised antigen

GAPDH Loading Control [Biotin Conjugate]

Host animal

Goat (Capra aegagrus hircus)

Clonality

Polyclonal (goat origin)

Species reactivity

Human (Homo sapiens), Mouse (Mus musculus), Rat, Pig ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.

Tested applications

WB, IHC, ICC, ELISA (peptide)

Recommended dilutions

Western blot: 0.3-1ug/ml,Immunohistochemistry (FFPE): suitable,Immunocytochemistry: suitable,ELISA (peptide) LOD: 1:64000

Notes

Optimal dilution of the GAPDH loading control antibody should be determined by the researcher.1. Do not use a blocking agent or diluent that contains biotin (including milk and some BSA preparations) when testing with biotinylated antibodies. A non-animal protein (NAP) blocker is recommended.

Intented use

This GAPDH loading control antibodyis to be used only for research purposes and not for diagnostics..

Gene ID #

2597

Purity

Antigen affinity

Description

Additional name(s) for this target protein: Glyceraldehyde-3-phosphate dehydrogenase; GAPDH

Immunogen

Amino acids HQVVSSDFNSDT were used as the immunogen for this GAPDH loading control antibody.

Storage

Aliquot and store the GAPDH loading control antibody at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.

Tissue

control

Properties

If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Additional conjugation

Biotinylated

Additional description

Isotype or positive controls by peptides, antibodies and deactivated samples.

French translation

anticorps