0.5mg/ml if reconstituted with 0.2ml sterile DI water
Antigen affinity purified
GAPDH Loading Control
Rabbit (Oryctolagus cuniculus)
Polyclonal (rabbit origin)
Human (Homo sapiens), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
Western blot: 0.5-1ug/ml
Titration of the GAPDH loading control antibody may be required due to differences in protocols and secondary/substrate sensitivity.
This GAPDH loading control antibodyis to be used only for research purposes and not for diagnostics..
Gene ID #
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. GAPDH is mapped to 12p13.31. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD).GAPDH antibody is commonly used as a western blot loading control.
Amino acids YSNRVVDLMAHMASKE were used as the immunogen for this GAPDH loading control antibody.
After reconstitution, the GAPDH loading control antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
Nuclear and cytoplasmic
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Isotype or positive controls by peptides, antibodies and deactivated samples.