Category

Antibody

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Form

Antigen affinity purified

Conjugation

Unconjugated

Clone

Polyclonal antibody

Recognised antigen

GAPDH Loading Control

Host animal

Rabbit (Oryctolagus cuniculus)

Clonality

Polyclonal (rabbit origin)

Species reactivity

Human (Homo sapiens), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.

Tested applications

WB

Recommended dilutions

Western blot: 0.5-1ug/ml

Notes

Titration of the GAPDH loading control antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Intented use

This GAPDH loading control antibodyis to be used only for research purposes and not for diagnostics..

Gene ID #

2597

Purity

Antigen affinity

Description

Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. GAPDH is mapped to 12p13.31. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD).GAPDH antibody is commonly used as a western blot loading control.

Immunogen

Amino acids YSNRVVDLMAHMASKE were used as the immunogen for this GAPDH loading control antibody.

Storage

After reconstitution, the GAPDH loading control antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.

Localization

Nuclear and cytoplasmic

Tissue

control

Properties

If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Additional description

Isotype or positive controls by peptides, antibodies and deactivated samples.

French translation

anticorps