0.5 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Beta Actin Loading Control
Rabbit (Oryctolagus cuniculus)
Polyclonal (rabbit origin)
Human (Homo sapiens), Mouse (Mus musculus), Rat, Monkey, Rabbit (Oryctolagus cuniculus), Chicken, Dog ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
Western blot: 1-3ug/ml
Provided assay concentrations are suggestions only, beta Actin antibody titration may be required for optimal results.
1X Phosphate Buffered Saline (PBS), pH 7.4
This beta Actin antibody loading controlis to be used only for research purposes and not for diagnostics..
Gene ID #
Purified by protein A Affinity Chromatography
Actins proteins are highly conserved and involved in cellular structure, integrity and motility. There are three main isoforms of actin in vertebrates: alpha, beta and gamma. Alpha actins are found in muscle cells while beta and gamma actins are non-muscle actins. Beta actin is commonly used as a loading control protein in western blot. An antibody to beta actin is used to demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.
A recombinant protein fragment from the N-terminus of human beta Actin was used as the immunogen for this antibody.
Aliquot the loading control antibody and store frozen at -20oC or colder to avoid repeated freeze-thaw cycles.
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Isotype or positive controls by peptides, antibodies and deactivated samples.