Beta Actin Antibody Loading Control

General information
 

Name:
Beta Actin Antibody Loading Control
Size:
0.025mg
Catalog no:
N1037-25UG
Price:
176 EUR
 

Additional extra details
 

  • Category

    Antibody

    Concentration

    0.5 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

    Form

    Purified

  • Conjugation

    Unconjugated

    Clone

    Polyclonal antibody

    Recognised antigen

    Beta Actin Loading Control

  • Host animal

    Rabbit (Oryctolagus cuniculus)

    Clonality

    Polyclonal (rabbit origin)

    Species reactivity

    Human (Homo sapiens), Mouse (Mus musculus), Rat, Monkey, Rabbit (Oryctolagus cuniculus), Chicken, Dog ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.

  • Tested applications

    WB

    Recommended dilutions

    Western blot: 1-3ug/ml

    Notes

    Provided assay concentrations are suggestions only, beta Actin antibody titration may be required for optimal results.

  • Added buffer

    1X Phosphate Buffered Saline (PBS), pH 7.4

    Intented use

    This beta Actin antibody loading controlis to be used only for research purposes and not for diagnostics..

    Gene ID #

    60

  • Purity

    Purified by protein A Affinity Chromatography

    Description

    Actins proteins are highly conserved and involved in cellular structure, integrity and motility. There are three main isoforms of actin in vertebrates: alpha, beta and gamma. Alpha actins are found in muscle cells while beta and gamma actins are non-muscle actins. Beta actin is commonly used as a loading control protein in western blot. An antibody to beta actin is used to demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.

    Immunogen

    A recombinant protein fragment from the N-terminus of human beta Actin was used as the immunogen for this antibody.

  • Storage

    Aliquot the loading control antibody and store frozen at -20oC or colder to avoid repeated freeze-thaw cycles.

    Properties

    If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

    Additional description

    Isotype or positive controls by peptides, antibodies and deactivated samples.

  • French translation

    anticorps