0.5 mg/ml in 1X PBS with 0.05% sodium azide
Rabbit IgG Isotype Control FITC Conjugate
Rabbit (Oryctolagus cuniculus)
Polyclonal (rabbit origin)
isotype control Igs
FACS, IHC, IF
FACS: assay dependent,IHC: assay dependent,IF: assay dependent
Generally to be used at the same concentration as the sample being controlled for.
1X Phosphate Buffered Saline (PBS), pH 7.4
This rabbit isotype control antibodyis to be used only for research purposes and not for diagnostics..
Gene ID #
Purified by protein A Affinity Chromatography
Isotype control antibodies are negative controls used to measure non-specific binding or background levels of a primary antibody in different cell types. They are not specific to any target in a cell, yet retain all non-specific characteristics of the antibodies they control.
This is purified native rabbit sera and as such there was no immunogen.
Store the Rabbit IgG isotype control antibody at 4oC. Do not freeze; protect from light.
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This NJS poly Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. NJS poly FITC is highly sensitive to pH extremes.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
Isotype or positive controls by peptides, antibodies and deactivated samples.
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by NJS poly. Rabbit antibodies are very stable and can be stored for several days at room temperature. NJS poly adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
isotypes,Polyclonals and antibodies