Category
Biological Reagents
Antibody Subtype
Isotype Controls
Area of research
Immunology
Raised in
Mouse
Isotypes
IgG2b kappa
Method of Purification
IgG2b Isotype Control Fc fusion protein was purified by affinity chromatography.
Concentration
500 ug/ml
Form & Buffer
Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
Storage
Store at 2-8 deg C. Do not freeze. Protect from light.
Shipping conditions
Blue Ice
Tested for
IHC; Functional Assay; IP; WB
Assay Information
This eBioBV14 (BV14) antibody has been tested by western blot analysis of bEnd.3 cell lysates, and can be used in western blotting at a starting concentration of 2 ug/ml
Tissue
control
Description
Isotype or positive controls by peptides, antibodies and deactivated samples.Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.
Properties
Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
Group
isotypes