Category

Biological Reagents

Antibody Subtype

Isotype Controls

Area of research

Immunology

Raised in

Rat

Isotypes

IgG2b kappa

Method of Purification

IgG2b Isotype Control Fc fusion protein was purified by affinity chromatography.

Concentration

500 ug/ml

Form & Buffer

Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.

Storage

Store at 2-8 deg C. Do not freeze. Protect from light.

Shipping conditions

Blue Ice

Tested for

FC; Functional Assay; IP

Usage Recommendations

FC: ≤ 0.5 ug/test

Assay Information

A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.

Tissue

control

Description

Isotype or positive controls by peptides, antibodies and deactivated samples.Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.

Properties

Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

Group

isotypes