Category

Biological Reagents

Antibody Subtype

Isotype Controls

Area of research

Immunology

Raised in

Mouse

Isotypes

IgG1 Kappa

Conjugate/Tag

Biotin

Form & Buffer

Provided in an aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer

Storage

Store at 2-8 deg C. Do not freeze.

Shipping conditions

Blue Ice

Tested for

FC; ICC; IHC

Tissue

control

Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

Conjugation

Biotinylated

Description

Isotype or positive controls by peptides, antibodies and deactivated samples.

Group

isotypes

French translation

anticorps