Category

Biological Reagents

Antibody Subtype

Isotype Controls

Area of research

Immunology

Raised in

Rat

Isotypes

IgG1 kappa

Concentration

500 ug/ml

Form & Buffer

Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.

Storage

Store at 2-8 deg C. Do not freeze. Protect from light.

Shipping conditions

Blue Ice

Tested for

FC

Assay Information

control has been tested by immunofluorescent staining and flow cytometric analysis of mouse splenocyte suspensions and can be used at the same concentration as the experimental antibody

Tissue

control

Description

Isotype or positive controls by peptides, antibodies and deactivated samples.Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.

Properties

This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

Conjugation

Anti-FITC Antibody

Group

isotypes