Category

Biological Reagents

Antibody Subtype

Isotype Controls

Area of research

Immunology

Raised in

Hamster

Specificity

Human

Isotypes

IgG

Clone

299Arm

Conjugate/Tag

Biotin

Concentration

500 ug/ml

Form & Buffer

Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.

Storage

Store at 2-8 deg C. Do not freeze. Protect from light.

Shipping conditions

Blue Ice

Tested for

FC

Usage Recommendations

FC: ≤ 0.25 ug/test

Assay Information

A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest

Tissue

control

Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

Conjugation

Biotinylated

Description

Isotype or positive controls by peptides, antibodies and deactivated samples.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG

Group

isotypes

French translation

anticorps