Description

Mouse IgG2a Human Isotype Control Monoclonal Antibody [B12/8]-CF-Blue™ is a mouse IgG2a monoclonal antibody type human isotype control, produced by [B12/8] clones, this reagent was conjugated with CF-Blue™ format and reported for use in flow cytometric analysis as a negative control, it's used for testing the reliabilty of biological tests. Use the same concetration of the specific antibody of interest.

Clone

B12/8

Conjugate

CF-Blue™

Application

Flow Cytometry Method

Formulation

Phosphate buffred solution, 0,09% sodium Azide, may contain carrier protein-stabilizer

Expiration

After 1 year

Storage

2-8°C, store in dark, do not freeze

Remark

For Recherche Use Only.

Tissue

control

Properties

If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

Conjugation

Blue Substrate

Additional description

Isotype or positive controls by peptides, antibodies and deactivated samples.

Additional isotype

IgG2a

Group

isotypes

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus

French translation

anticorps