Clone

RTK2071

Conjugated

FITC

Tested application

Flow Cytometry

Long description

This FITC-Conjugated Rat IgG1 Isotype Control was affinity purified from rabbit serum. It does not cross-react with any human or animal antigen

Storage buffer

PBS, 0,09% sodium Azide, may contain carrier protein-stabilizer

Shipping conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Technical datasheet

Please contact us to receive the latest datasheet, the certificate of analysis or the MSDS

Regulation status

This antibody is intended for Research Use Only.

Description

Isotype or positive controls by peptides, antibodies and deactivated samples.

Properties

This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

Conjugation

Anti-FITC Antibody

Additional isotype

IgG1

Group

isotypes

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Latin name

Rattus norvegicus