Target antigen

IgG2a Isotype Control

Clone

C1.18

Conjugation/additive

FITC Conjugated

Product type

Antibody

Clonality

monoclonal antibody

Long name

IgG2a Isotype Control Monoclonal Antibodies [C1.18], FITC Conjugated

Description

IgG2a Isotype Control. Monoclonal antibodies ensure a highly specific binding to a certain epitope of the antigen and are much more LOT-to-LOT consistent than polyclonal antibodies.

Storage conditions

For short term storage the IgG2a Isotype Control Monoclonal Antibody [C1.18], FITC Conjugated can be kept refrigerated at +4 degrees Celsius. For long term storage keep frozen at -20 degrees Celsius. Be sure to refer to the technical sheet for any additional specifications and requirements for proper storing of the antibodies.

Tips

IgG2a Isotype Control MAb [C1.18], FITC Conjugated should not be exposed to cycles of freezing and thawing as they may denaturate the peptide chains/bonds in the antibodies and reduce/change the activity of the antibody. Prior to use briefly centrifuge the vial to collect its content on the bottom.

Tissue

control

Properties

If you buy Antibodies supplied by Epigen they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Epigen Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Epigen FITC is highly sensitive to pH extremes.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

Additional description

Isotype or positive controls by peptides, antibodies and deactivated samples.

Conjugation

Anti-FITC Antibody

Group

isotypes

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

French translation

anticorps