Category

Other Reagents

Long description

EDG6 (S1P4) belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG6 (S1P4) is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG6 (S1P4) is expressed in lymphoid and hematopoietic tissue and in the lungs.

Antibody come from

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with human HIF-1 (amino acids 432-528) protein and mouse myeloma NS1 cells.

Other description

Provided as solution in phosphate buffered saline with 0.08% sodium azide.

Clone

not specified

Antigen-antibody binding interaction

Sphingosine 1 Phosphate 4 Receptor (CT) (EDG6) - Control Lysate

Antibody is raised in

see techfile

Antibody's reacts with

N/A

Antibody's reacts with these species

This antibody doesn't cross react with other species

Antibody's specificity

No Data Available

Application

Western Blot

Antibody's suited for

This antibody can be used for immunoprecipitation (2 µg/mg of protein lysate), Western blotting (0.5-1.0 µg/ml) and immunohistochemistry on frozen and formalin/paraffin tissue sections (2-4 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Storage

-20ºC

Relevant references

1. Flamme I; et al. HRF, a putative basic helix-loop-helix-PAS-domain transcription factor is closely related to hypoxia-inducible factor-1 alpha and developmentally expressed in blood vessels. Mechanisms of Development, 1997, 63(1):51-60._x000B__x000B_2. Gassmann M; et al. Oxygen- and dioxin-regulated gene expression in mouse hepatoma cells. Kidney International, 1997 Feb, 51(2):567-74._x000B__x000B_3. Huang LE; et al. Erythropoietin gene regulation depends on heme-dependent oxygen sensing and assembly of interacting transcription factors. Kidney International, 1997 Feb, 51(2):548-52._x000B__x000B_4. Jelkmann W; et al. Effects of antioxidant vitamins on renal and hepatic erythropoietin production. Kidney International, 1997 Feb, 51(2):497-501._x000B__x000B_5. Pugh CW; et al. Activation of hypoxia-inducible factor-1; definition of regulatory domains within the alpha subunit. Journal of Biological Chemistry, 1997, 272(17):11205-14._x000B__x000B_6. Semenza GL; et al. Structural and functional analysis of hypoxia-inducible factor 1. Kidney International, 1997, 51(2):553-5._x000B__x000B_7. Arany Z; et al. An essential role for p300/CBP in the cellular response to hypoxia. Proceedings of the National Academy of Sciences of the United States of America, 1996, 93(23):12969-73._x000B__x000B_8. Zhong, H., et al., Overexpression of Hypoxia-inducible Factor 1a in common human cancers and their metastases. Cancer Research 1999, 59, 5830-5835._x000B__x000B_9. Semenza, GL, et al. J. Lab Clinical Medicine. 131: 207-214, 1998. _x000B__x000B_10. Acs, G, et al. Am. J. Pathol. 162(6): 1789-1806, 2003.

Protein number

see ncbi

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Tissue

control

Description

Isotype or positive controls by peptides, antibodies and deactivated samples.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.