Category
Other Reagents
Long description
Total cell Iysates are useful as both positive and negative controls in immunoblotting. A431, a human epidermoid carcinoma cell line, and the EGF-stimulated A431 Iysates are used as negative and positive controls, respectively, when studying the phosphorylation cascade initiated by ligand binding to receptor tyrosine kinases. Both A431 and normal human fibroblasts are valuable positive controls for antibodies to proteins expressed in these cells. The mouse fibroblast cell line 3T3 and its RSV-transformed counterpart serve as negative and positive controls, respectively, for antibodies to proteins that are phosphorylated as a result of transformation by RSV (Rous sarcoma virus). Madin-Darby bovine kidney (MDBK) cell Iysate displays proteins expressed in bovine kidney and PC12 (rat phaeochromocytoma) cell Iysate can be expected to mirror the protein expression of cells and tissues of neuroectodermal origin. Likewise, Jurkat cells express the proteins unique to human T-cells. Rat brain is a widely used positive control for those proteins that are exclusively expressed in brain. At right is an example of the use of positive and negative control Iysates in western blotting. _x000B__x000B_The blot of 3T3 cell Iysate (lane 1) and RSV-transformed 3T3 (lane 2) was probed with anti-phosphotyrosine peroxidase conjugate to reveal those proteins that are tyrosine-phosphorylated in response to transformation by Rous sarcoma virus.
Antibody come from
Hybridoma produced by the fusion of spelocytes from BALB/c mice immunized with recombinant extracellular domain of rat NDF protein coupled to KLH and mouse myeloma NSO cells.
Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide.
Clone
not specified
Antigen-antibody binding interaction
HeLa antibody Control Lysate
Antibody is raised in
see techfile
Antibody's reacts with
see techfile
Antibody's reacts with these species
This antibody doesn't cross react with other species
Antibody's specificity
No Data Available
Application
Western Blot
Antibody's suited for
This antibody can be used for Western blotting (1-5 µg/ml), immunoprecipitation (2 µg/mg of protein lysate) and immunohistochemistry on frozen and formalin/paraffin tissue sections (1:20-1:40 dilution). Optimal concentration should be evaluated by serial dilutions.
Storage
-20ºC
Relevant references
1. Graus-Porta D; et al. Single-chain antibody-mediated intracellular retention of ErbB-2 impairs Neu differentiation factor and epidermal growth factor signaling. Molecular and Cellular Biology, 1995, 15(3):1182-91._x000B__x000B_2. Devarajan K, et al. (1996) ErbB-2 is a common auxiliary subunit of NDF- and EGF-receptors: implications for breast cancer. EMBO. J. 15 : 254-264._x000B__x000B_3. Marte BM; et al. NDF/heregulin activates MAP kinase and p70/p85 S6 kinase during proliferation or differentiation of mammary epithelial cells. Oncogene, 1995, 10(1):167-75._x000B__x000B_4. Beerli, R.R., et al. 1995. Neu Differentiation Factor activation of ErbB-3 and ErbB-4 is cell specific and displays a differential requirement for ErbB-2. Mol. Cell. Biol., 15:6496-6505._x000B__x000B_5. Lupu, R., et al. (1995) Seminars in Cancer Biology, 6:135- 145._x000B__x000B_6. Holmes W, et al. (1992)Science 256:1205-1210. _x000B__x000B_7. Peles E, et al. (1992) Cell 69:205-216.
Protein number
see ncbi
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Tissue
control
Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
Isotype or positive controls by peptides, antibodies and deactivated samples.
French translation
anticorps