Category

Other Reagents

Long description

Monocyte chemotactic protein (MCP-1) is also known as monocyte chemotactic and activating factor (MCAF). It is the product of the human JE gene. The precursor form of MCP-1 consists of 99 amino acids with a signal peptide sequence consisting of 23 amino-terminal amino acids. The mature form of MCP-1 has 4 cysteine residues. The first two cysteine residues are in an adjacent position C-C, which characterizes MCP-1 as a member of the chemokine subfamily. MCP-1 is a nonglycosylated protein consisting of 76 amino acids with a molecular weight of 8700 daltons. MCP-1 mRNA expression can be induced in monocytes/macrophages, B lymphocytes, endothelial cells and astrocytoma cells by LPS. IL-1 will induce production of MCP-1 in fibroblasts, keratinocytes, hepatoma cells, and Type II pneumocytes. In vitro, MCP-1 will act on monocytes to initiate chemotaxis, induce superoxide anion release, induce the release of lysosomal enzymes and augment cytostatic activity. In vivo, MCP-1 will induce macrophage infiltration. The MCP-1 gene contains potential binding sites for several transcription factors, including AP-1, AP-2, NF-kB, and NF-IL6.

Antibody come from

Hybridoma produced by the fusion of splenocytes from mice immunized with purified human keratin 7 isolated from HeLa cytoskeleton and mouse myeloma cells.

Other description

Provided as solution in phosphate buffered saline with 0.08% sodium azide.

Clone

not specified

Antigen-antibody binding interaction

MCP-1 - Control Lysate

Antibody is raised in

see techfile

Antibody's reacts with

see techfile

Antibody's reacts with these species

This antibody doesn't cross react with other species

Antibody's specificity

No Data Available

Application

Activity Assay

Antibody's suited for

Detects keratin 7 by Western blot. Detects a 54 kDa band corresponding to keratin 7. Optimal concentration should be evaluated by serial dilutions.

Storage

-20ºC

Relevant references

1. Neoplasma 1990, 37, 333-342_x000B__x000B_2. J. Tumor Marker Oncol. 1990, 5, 219_x000B__x000B_3. Int. J. Cancer 1988, Suppl. 3, 50-55

Protein number

see ncbi

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Tissue

control

Description

Isotype or positive controls by peptides, antibodies and deactivated samples.