Monocyte chemotactic protein (MCP-1) is also known as monocyte chemotactic and activating factor (MCAF). It is the product of the human JE gene. The precursor form of MCP-1 consists of 99 amino acids with a signal peptide sequence consisting of 23 amino-terminal amino acids. The mature form of MCP-1 has 4 cysteine residues. The first two cysteine residues are in an adjacent position C-C, which characterizes MCP-1 as a member of the chemokine subfamily. MCP-1 is a nonglycosylated protein consisting of 76 amino acids with a molecular weight of 8700 daltons. MCP-1 mRNA expression can be induced in monocytes/macrophages, B lymphocytes, endothelial cells and astrocytoma cells by LPS. IL-1 will induce production of MCP-1 in fibroblasts, keratinocytes, hepatoma cells, and Type II pneumocytes. In vitro, MCP-1 will act on monocytes to initiate chemotaxis, induce superoxide anion release, induce the release of lysosomal enzymes and augment cytostatic activity. In vivo, MCP-1 will induce macrophage infiltration. The MCP-1 gene contains potential binding sites for several transcription factors, including AP-1, AP-2, NF-kB, and NF-IL6.
Antibody come from
Hybridoma produced by the fusion of splenocytes from mice immunized with purified human keratin 7 isolated from HeLa cytoskeleton and mouse myeloma cells.
Provided as solution in phosphate buffered saline with 0.08% sodium azide.
Antigen-antibody binding interaction
MCP-1 - Control Lysate
Antibody is raised in
Antibody's reacts with
Antibody's reacts with these species
This antibody doesn't cross react with other species
No Data Available
Antibody's suited for
Detects keratin 7 by Western blot. Detects a 54 kDa band corresponding to keratin 7. Optimal concentration should be evaluated by serial dilutions.
1. Neoplasma 1990, 37, 333-342_x000B__x000B_2. J. Tumor Marker Oncol. 1990, 5, 219_x000B__x000B_3. Int. J. Cancer 1988, Suppl. 3, 50-55
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Isotype or positive controls by peptides, antibodies and deactivated samples.