Category

Other Reagents

Long description

Leukotactin-1 (Lkn-1), also known as Macrophage inflammatory protein 1 delta (MIP-1 ), MIP-5, HCC-2, and NCC-3, is a novel human CC chemokine whose gene was mapped to human chromosome 17 adjacent to the HCC-1 gene. Lkn-1, together with mouse C10, mouse MIP-1 and human MPIF-1, constitute a subgroup of CC chemokines which contain six instead of four conserved cysteine residues. The two additional cysteine residues in Lkn-1 have been shown to form a third disulfide bond. Lkn-1 cDNA encodes a 113 amino acid precursor protein with a putative signal peptide of 21 aa residues that is cleaved to generate a 92 amino acid mature protein. In recombinant Lkn-1 preparations produced in insect cells and in yeast, amino-terminal truncations were found to have occurred. The major forms of Lkn-1 secreted by insect cells and yeast were reported to be proteins of 68 and 66 amino acids, respectively. The full length and the amino-terminal truncated forms of human Lkn-1 have been shown to be potent chemoattractants for monocytes and T-lymphocytes. These proteins can also chemoattract eosinophils and have been shown to induce calcium flux in human CCR-1 transfected cells. Additionally, Lkn-1 can suppress colony formation by human granulocyte-macrophage, erythroid, and multipotential progenitor cells stimulated by combinations of growth factors.

Antibody come from

Hybridoma produced by the fusion of splenocytes from mice immunized with cytoskeleton preparation of HeLa cells and mouse myeloma cells.

Other description

Provided as solution in phosphate buffered saline with 0.08% sodium azide.

Clone

not specified

Antigen-antibody binding interaction

Leukotactin/Mip-5 - Control Lysate

Antibody is raised in

see techfile

Antibody's reacts with

Human

Antibody's reacts with these species

This antibody doesn't cross react with other species

Antibody's specificity

No Data Available

Application

Activity Assay

Antibody's suited for

Detects Keratin 5 & 8 by Western blot. Detects a 58 kDa band, corresponding to Keratin 5, and a 52.5 kDa band, corresponding to Keratin 8. Optimal concentration should be evaluated by serial dilutions.

Storage

-20ºC

Relevant references

1. Kovarik, et al, J. Tumor Marker Oncol. 1990, 5, 219

Protein number

see ncbi

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Tissue

control

Description

Isotype or positive controls by peptides, antibodies and deactivated samples.