Category

Other Reagents

Long description

Fractalkine, also termed neurotactin, is a membrane-bound CX3C chemokine. The mature protein is part of a 397-amino acid precursor consisting of a chemokine domain (76 amino acids), a mucin stalk of 241 residues, a putative transmembrane domain (18 amino acids), and an intracellular tail of 37 amino acids. Within the chemokine domain the first two cysteine residues are separated by 3 amino acids. Fractalkine message is found at high concentrations in the brain, and also in kidney, lung and heart. Fractalkine is chemotactic for monocytes and may play a role in brain inflammation. Product provided is the 76 amino acid chemokine domain with a C-terminal biotin.

Antibody come from

Hybridoma produced by the fusion of splenocytes from mice immunized with synthetic peptide conjugated to KLH corresponding to the carboxy terminal sequence of human cytokeratin 14 (KVVSTHEQVLRTKN) and mouse myeloma cells.

Other description

Provided as solution in phosphate buffered saline with 0.08% sodium azide.

Clone

not specified

Antigen-antibody binding interaction

Fractalkine-Biotin - Control Lysate

Antibody is raised in

see techfile

Antibody's reacts with

see techfile

Antibody's reacts with these species

This antibody doesn't cross react with other species

Antibody's specificity

No Data Available

Application

Activity Assay

Antibody's suited for

Detects human cytokeratin 14 by Western blot at 50 kDa. Does not react with other cytokeratins or other proteins. Also works for immunohistochemistry using frozen tissues. Does not work with paraffin embedded tissues. Optimal concentration should be evaluated by serial dilutions.

Storage

-20ºC

Relevant references

1. Moll, R., et al. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982, 31, 11-24_x000B__x000B_2. Ivanyi, D., et al. Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells. American Journal of Veterinary Research, 1992, 53, 304-314.

Protein number

see ncbi

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Tissue

control

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Conjugation

Biotinylated

Description

Isotype or positive controls by peptides, antibodies and deactivated samples.