Fractalkine-Biotin - Control Lysate

General information
 

Name:
Fractalkine-Biotin - Control Lysate
Size:
20 μg
Catalog no:
X1239C
Price:
532 EUR
 

Additional extra details
 

  • Category

    Other Reagents

    Long description

    Fractalkine, also termed neurotactin, is a membrane-bound CX3C chemokine. The mature protein is part of a 397-amino acid precursor consisting of a chemokine domain (76 amino acids), a mucin stalk of 241 residues, a putative transmembrane domain (18 amino acids), and an intracellular tail of 37 amino acids. Within the chemokine domain the first two cysteine residues are separated by 3 amino acids. Fractalkine message is found at high concentrations in the brain, and also in kidney, lung and heart. Fractalkine is chemotactic for monocytes and may play a role in brain inflammation. Product provided is the 76 amino acid chemokine domain with a C-terminal biotin.

    Antibody come from

    Hybridoma produced by the fusion of splenocytes from mice immunized with synthetic peptide conjugated to KLH corresponding to the carboxy terminal sequence of human cytokeratin 14 (KVVSTHEQVLRTKN) and mouse myeloma cells.

  • Other description

    Provided as solution in phosphate buffered saline with 0.08% sodium azide.

    Clone

    not specified

    Antigen-antibody binding interaction

    Fractalkine-Biotin - Control Lysate

  • Antibody is raised in

    see techfile

    Antibody's reacts with

    see techfile

    Antibody's reacts with these species

    This antibody doesn't cross react with other species

  • Antibody's specificity

    No Data Available

    Application

    Activity Assay

    Antibody's suited for

    Detects human cytokeratin 14 by Western blot at 50 kDa. Does not react with other cytokeratins or other proteins. Also works for immunohistochemistry using frozen tissues. Does not work with paraffin embedded tissues. Optimal concentration should be evaluated by serial dilutions.

  • Storage

    -20ºC

    Relevant references

    1. Moll, R., et al. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982, 31, 11-24_x000B__x000B_2. Ivanyi, D., et al. Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells. American Journal of Veterinary Research, 1992, 53, 304-314.

    Protein number

    see ncbi

  • Warnings

    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

    Tissue

    control

    Properties

    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

  • Conjugation

    Biotinylated

    Description

    Isotype or positive controls by peptides, antibodies and deactivated samples.