BRAK is a novel member of the CXC chemokine family. The function of BRAK in cells remains largely unknown. It was identified by translating expressed sequence tags (ESTs) derived from breast and kidney carcinoma libraries and was found to be expressed ubiquitously in RNA from normal tissue extracts but absent from a variety of established in vitro tumor cell lines.
Antibody come from
Hybridoma produced by the fusion of splenocytes from mice immunized with cytoskeleton preparation from HeLa cells and mouse myeloma cells.
Provided as solution in phosphate buffered saline with 0.08% sodium azide.
Antigen-antibody binding interaction
Brak-Biotin - Control Lysate
Antibody is raised in
Antibody's reacts with
Antibody's reacts with these species
This antibody doesn't cross react with other species
No Data Available
Antibody's suited for
Antibody detects cytokeration protein numbers 13 and 10 (molecular weights 53 and 56.6 kDa, respectively, according to Moll's classification) by Western blot. Does not react with other cytokeratins or other proteins. Optimal concentration should be evaluated by serial dilutions.
1. Ivanyi, D., et al. Modulation of mammary carcinoma cell phenotype and keratin expression patterns by retinoic acid. Cancer Lett, 1993, 73, 191-205._x000B__x000B_2. Ivanyi, D., et al. Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas. Am. J. Vet. Res. 1993, 54, 1095-1102._x000B__x000B_3. Ivanyi, D., et al. Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells. American Journal of Veterinary Research, 1992, 53, 304-314._x000B__x000B_4. Ivanyi, D., et al. Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis. Cancer Research, 1990, 50, 5143-5152._x000B__x000B_5. Ivanyi, D., et al. New monoclonal antibodies recognizing epidermal differentiation-associated keratins in formalin-fixed, paraffin-embedded tissue. Keratin 10 expression in carcinoma of the vulva. J of Pathol, 1989, 159, 7-12._x000B__x000B_6. Moll, R., et al. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982, 31, 11-24
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Isotype or positive controls by peptides, antibodies and deactivated samples.