Category

Negative Control

Long description

Negative control for Immunofluorescence staining with FITC and PE of mouse monoclonal antibodies. Assessment of non-specific binding of mouse monoclonal antibodies to human cell surface antigens.

Antibody come from

n/a

Other description

Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography

Clone

not specified

Antigen-antibody binding interaction

Mouse IgG1 antibody conjugated to FITC - IgG2a antibody conjugated to PE - Negative Control

Antibody is raised in

Mouse

Antibody's reacts with

see techfile

Antibody's reacts with these species

This antibody doesn't cross react with other species

Antibody's specificity

No Data Available

Application

Flow Cytometry

Antibody's suited for

Consult the appropriate fact sheet to determine the amount of antibody to be used as a control for PBMC or Whole Blood. PBMC: Gently add10 µl of MAB/10^6 PBMC in100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼ C. Wash twice with PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of Whole Blood. Mix gently and incubate for 15 minutes at room temperature 20¼C. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Storage

4ºC

Relevant references

no information yet

Protein number

see ncbi

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Properties

If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.

Description

Isotype or positive controls by peptides, antibodies and deactivated samples.Negative control peptides or isotypes available of the different species.

Conjugation

Anti-FITC Antibody

Additional isotype

IgG1,IgG2a

French translation

anticorps