Mouse IgG2a Isotype Control

General information
 

Name:
Mouse IgG2a Isotype Control
Size:
25ug
Catalog no:
1A-724-C025
Price:
131 EUR
 

Additional extra details
 

  • Long name

    Mouse Monoclonal IgG2a Isotype Control

    Antigen

    Mouse IgG2a Isotype Control

    Species reactivity

    Immunoglobulin Isotype Control

  • Negative species reactivity

    Human, Mouse, Rat

    Clonality

    Monoclonal Antibody (Mab)

    Clone

    MOPC-173

  • Applications

    FC, IP, WB, IHC, ICC, negative control

    Immunogen

    The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.

    Format/conjugation

    APC

  • Specificity

    This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.

    Research area

    Immunoglobulin Isotype Controls

    Concentration

    0.1 mg/ml

  • Storage buffer

    The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.

    Shipping and storage

    Mouse Monoclonal IgG2a Isotype Control is shipped on blue ice or ice packs. Upon receipt store at 4 degrees Celcius. Do not freeze!

    Background

    The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

  • Purity

    > 95% (by SDS-PAGE)

    Purification method

    Purified by affinity chromatography

    References

    *Khoddami V, Cairns BR: Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP. Nat Protoc. 2014 Feb;9(2):337-61., *Gupta V, Gylling A, Alonso JL, Sugimori T, Ianakiev P, Xiong JP, Arnaout MA: The beta-tail domain (betaTD) regulates physiologic ligand binding to integrin CD11b/CD18. Blood. 2007 Apr 15;109(8):3513-20., *Baumal R, Scharff MD: Immunoglobulin biosynthesis by the MOPC 173 mouse myeloma tumor and a variant spleen clone. J Immunol. 1976 Jan;116(1):65-74., *Fougereau M, Bourgois A, de Preval C, Rocca-Serra J, Schiff C: The complete sequence of the murine monoclonal immunoglobulin MOPC 173 (IgG2a): genetic implications. Ann Immunol (Paris). 1976 Sep-Oct;127(5):607-31., *And many other.

  • Description

    Isotype or positive controls by peptides, antibodies and deactivated samples.

    Additional isotype

    IgG2a

    Properties

    Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

  • Group

    isotypes

    Test

    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

    Latin name

    Mus musculus