Mouse IgG2b Isotype Control

General information
 

Name:
Mouse IgG2b Isotype Control
Size:
25ug
Catalog no:
1B-692-C025
Price:
116 EUR
 

Additional extra details
 

  • Long name

    Mouse Monoclonal IgG2b Isotype Control

    Antigen

    Mouse IgG2b Isotype Control

    Species reactivity

    Immunoglobulin Isotype Control

  • Negative species reactivity

    Human, Mouse, Rat, Other species Not tested

    Clonality

    Monoclonal Antibody (Mab)

    Clone

    MPC-11

  • Applications

    FC, IP, WB, IHC(P), IHC(F), ICC, negative control

    Immunogen

    KLH-coupled trinitrophenol

    Format/conjugation

    biotin

  • Specificity

    This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.

    Research area

    Immunoglobulin Isotype Controls

    Concentration

    1 mg/ml

  • Storage buffer

    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

    Shipping and storage

    Mouse Monoclonal IgG2b Isotype Control is shipped on blue ice or ice packs. Upon receipt store at 4 degrees Celcius. Do not freeze!

    Background

    The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

  • Purity

    > 95% (by SDS-PAGE)

    Purification method

    Purified by affinity chromatography

    References

    *And other., *Liang XS, Zhou Y, Li CZ, Wan MB: Natural course of chronic hepatitis B is characterized by changing patterns of programmed death type-1 of CD8-positive T cells. World J Gastroenterol. 2010 Feb 7;16(5):618-24., *Dewals BG, Vanderplasschen A: Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues. Vet Res. 2011 Aug 22;42(1):95., *Gupta D, Wang Q, Vinson C, Dziarski R: Bacterial peptidoglycan induces CD14-dependent activation of transcription factors CREB/ATF and AP-1. J Biol Chem. 1999 May 14;274(20):14012-20., *Smed-Sörensen A, Moll M, Cheng TY, Loré K, Norlin AC, Perbeck L, Moody DB, Spetz AL, Sandberg JK: IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008 May 15;111(10):5037-46. , *Im JS, Tapinos N, Chae GT, Illarionov PA, Besra GS, DeVries GH, Modlin RL, Sieling PA, Rambukkana A, Porcelli SA: Expression of CD1d molecules by human schwann cells and potential interactions with immunoregulatory invariant NK T cells. J Immunol. 2006 Oct 15;177(8):5226-35.

  • Description

    Isotype or positive controls by peptides, antibodies and deactivated samples.

    Additional isotype

    IgG2b

    Properties

    Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

  • Group

    isotypes

    Test

    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

    Latin name

    Mus musculus