Long name

Mouse Monoclonal IgG2a Isotype Control

Antigen

Mouse IgG2a Isotype Control

Species reactivity

Immunoglobulin Isotype Control

Negative species reactivity

Human, Other species Not tested (expected negative), Porcine, Mouse, Rat

Clonality

Monoclonal Antibody (Mab)

Clone

PPV-04

Applications

FC, negative control

Immunogen

Ig Control

Format/conjugation

purified, unconjugated

Specificity

This mouse IgG2a monoclonal antibody (clone PPV-04) reacts with undefined epitope on a plant pathogen.

Research area

Immunoglobulin Isotype Controls

Concentration

1 mg/ml

Storage buffer

Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Shipping and storage

Mouse Monoclonal IgG2a Isotype Control is shipped on blue ice or ice packs. Upon receipt store at 4 degrees Celcius. Do not freeze!

Background

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-04, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

Purity

> 95% (by SDS-PAGE)

Purification method

Purified by protein-A affinity chromatography

References

Contact us for the most recent list of references

Description

Isotype or positive controls by peptides, antibodies and deactivated samples.

Additional isotype

IgG2a

Properties

Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

Group

isotypes

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus