Available ordering format
Liquid
Immunogen
to be determined
Raised in
N/A
Clonality
N/A
Clone
N/A
Purification
Affinity purified
How to reconstitute
See included datasheet or contact our support service
Storage condition
store protein standard at -20°C; Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Verified applications
Western blot (WB)
Connected products
AS08 307 | UBQ11 | Ubiquitin antibodies
Recommended dilutions for use
positive control: a 2.5-5 μl load per well is optimal for most chemiluminescent detection systems.
Molecular weight (expected | аpparent)
10-12 kDa
Verified reactivity
to be determined
Possible reactivity
to be determined
No reactivity
no confirmed exceptions from predicted reactivity currently known
Supplementary information
Concentration: 0.8 µg/µl. Recommended load per gel to allow Coomasie staining and western blot detection is 2.5-5 µl.1x SDS Loading Buffer contains:50 mM Tris-HCl pH 6.82% SDS10% glycerol1% β-mercaptoethanol12.5 mM EDTA0.02% bromophenol blue
References
to be added when available
Scientific context
Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS).Source of positive control, ubiquitin protein: full length recombinant Arabidopsis thaliana ubiquitin, ubq11, with N-terminal 1XHis-3XHA-tag; locus accession: At4g05050
Notes
Protein is provided in Laemli SDS loading buffer and should be heated up in 98°C for 5 minutes before loading on the gel.
Protein number
Refer to NCBI
TAIR number
Refer to NCBI
Tissue
control
Description
Isotype or positive controls by peptides, antibodies and deactivated samples.Positive controls are the same as the target vector or antibody or protein and can be spiked to the sample before the analysis starts.
Group
positif