Available ordering format
Lyophilized
Immunogen
to be determined
Raised in
N/A
Clonality
N/A
Clone
N/A
Purification
Affinity purified
How to reconstitute
For reconstitution add 225 µl of milliQ water
Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Verified applications
western blot (WB)
Connected products
AS06 111 | anti-CP43' | IsiA homolog of plant CP43 antibodiesCollection of global antibodiesCollection of antibodies to photosynthetic proteins Plant and algal protein extraction buffer
Recommended dilutions for use
Standard curve: 3 loads are recommended (2.5 and 10 μl).For most applications a sample load of 0.2μg of chlorophyll will give a IsiA signal in this range.Positive control:a 2μl load per well is optimal for most chemiluminescent detection systems.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Molecular weight (expected | аpparent)
27 kDa (slightly larger than native protein due to His-tag)
Verified reactivity
to be determined
Possible reactivity
to be determined
No reactivity
to be determined
Supplementary information
Concentration: after adding 225 µl of milliQ water final concentration of the standard is 0.15 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
References
Fraser et al. (2013). Photophysiological and Photosynthetic Complex Changes during Iron Starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942.PLoS ONE 8(3): e59861. doi:10.1371/journal.pone.0059861Ryan-Keogh et al. (2012). Iron deficiency in cyanobacteria causes monomerization of photosystem I trimers and reduces the capacity for state transitions and the effective absorption cross section of photosystem I in vivo. J. of Phycology, 1:145-154.
Scientific context
This product is a recombinant protein standard, source: Synechocystis strain PCC 6803.
Notes
The IsiA protein standard can be used in combination with anti-IsiA antibodies to quantitate IsiA from a range of cyanobacteria. Global antibodies are raised against highly conserved amino acid sequences in theIsiA protein.Quantitative western blot: detailed method description, video tutorial
Protein number
Refer to NCBI
TAIR number
Refer to NCBI
Tissue
control
Description
Isotype or positive controls by peptides, antibodies and deactivated samples.Positive controls are the same as the target vector or antibody or protein and can be spiked to the sample before the analysis starts.
About
Plants RNA, DNA , cDNA and plant tissues are used to study plant genes.
Group
positif