Available ordering format
Lyophilized
Immunogen
KLH-conjugated synthetic peptide conserved in all known PsaC proteins includingArabidopsis thaliana AtCg01060, Hordeum vulgare P69416, Oryza sativa P0C360, Chlamydomonas reinhardtii Q00914, Synechococcus elongatus Q31QV2
Raised in
Rabbit
Clonality
Polyclonal
Clone
Polyclonal
Purification
Serum
How to reconstitute
See included datasheet or contact our support service
Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Verified applications
western blot (WB)
Connected products
AS04 042S | PsaC protein standard for quantitationcollection of antibodies to PSI proteinsPlant and algal protein extraction bufferSecondary antibodies
Recommended dilutions for use
1: 1000 with ECL (WB)
Molecular weight (expected | аpparent)
9 kDa
Verified reactivity
Arabidopsis thaliana, Horderum vulgare, Spinacia oleracea, Synechococcus PCC 7942, Cyanophora paradoxa, Heterosigma akashiwo, Thalassiosira pseudonan, Euglena gracilis, Micromonas pusilla, Chlamydomonas reinhardtii, Porphyra sp., Gonyaulax polyedra, Emiliania huxleyi
Possible reactivity
dicots including Glycine max, Nicotiana tabacum, Spinacia oleracea, and monocots, Physcomitrella patens, algae and cyanobacteria, Prochlorococcus sp. (surface and a deep water ecotype)
No reactivity
no confirmed exceptions from predicted reactivity known in the moment
Supplementary information
In some species minor cross reactions with some larger proteins are seen. These may contain related iron-sulfur binding motifs. Therefore size verification of the reacting band is required. Due to the small size of the protein, care should be taken to differentiate between chemiluminescent signal from PsaC and non-specific signals from chlotophylls or lipids if pigment is retained near the bottom of the blot.Protein standard: use a load of 2 µl per well with ECL detection system and 4 µl per well with alkaline phospatase.
References
Ifuku et al. (2005). PsbP protein, but not PsbQ protein, is essential for the regulation and stabilization of photosystem II in higher plants. Plant Physiol. 3:1175-1184. Oesterhelt et al (2007). Regulation of photosynthesis in the unicellular acidophilic red alga Galdieria sulphuraria. Plant J.3:500511.
Scientific context
PsaC is a conserved, chloroplast-encoded, Fe-S binding protein of approximately 10 kDa, present in all known Photosystem I complexes. It is located on the stromal side of the thylacoid membranes. PsaC coordinates the Fe-S clusters FA and FB through two cysteine-rich domains.
Notes
Peptide target used to elicit this antibody is well conserved in all photoautotrophs except some cyanobacteria, some red algae and Cyanophora paradoxa, which contain a conserved substitution of a valine to an isoleucine. The performance of the antibodies has been confirmed against taxa containing both the valine and isoleucine variants. For reconstitution of PsaC antibodies (AS10 939) add 50 µl of sterile water. For reconstitution of PsaC positive control add 100 µl of sterile water.
Protein number
P62090 , P69416 , P0C360 , Q00914 , Q31QV2
TAIR number
ATCG01060
Tissue
control
Properties
If you buy Antibodies supplied by agisera they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
Isotype or positive controls by peptides, antibodies and deactivated samples.Positive controls are the same as the target vector or antibody or protein and can be spiked to the sample before the analysis starts.
Group
positif
French translation
anticorps