Category

Isotype Control

Clonality

Monoclonal

Raised in

Mouse

Target

Mouse IgG2a Isotype Control

Immunogen

see included datasheet or contact us

Species reactivity

Human, Mouse, Rat (for other species please inquire)

Purification

Affinity purified

Applications

WB (for other applications please contact us)

Stock availability

Shipped within 5-12 working days.

Storage conditions

Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.

Footnote

This product is for research use only.

Tissue

control

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

Conjugation

Biotinylated

Description

Isotype or positive controls by peptides, antibodies and deactivated samples.

Group

isotypes

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus