Category
Isotype Control
Clonality
Monoclonal
Raised in
Rat
Target
Rat IgG2a Isotype Control
Immunogen
see included datasheet or contact us
Species reactivity
Human, Mouse, Rat (for other species please inquire)
Purification
Affinity purified
Applications
WB (for other applications please contact us)
Stock availability
Shipped within 5-10 working days.
Storage conditions
Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Footnote
This product is for research use only.
Tissue
control
Description
Isotype or positive controls by peptides, antibodies and deactivated samples.
Properties
This abbex Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. abbex FITC is highly sensitive to pH extremes.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
Conjugation
Anti-FITC Antibody
Group
isotypes
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus