For short term storage Interleukin-6 Soluble Receptor (Western Blot Control) Protein may be kep at +4 deg. C. For long term storage keep the aliquoted vials frozen at -20 degrees Celsius.
Due to handling and transportation, small amounts of the Interleukin-6 Soluble Receptor (Western Blot Control) Protein may get caught on the walls or seal of the vials. Prior to use briefly rund the vial(s) through a centrifuge to collect all of the content on the bottom. Avoid freezing and thawing the vials numerous times as such cycles may denaturate the peptide chains and influence the item's performance.
Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
Isotype or positive controls by peptides, antibodies and deactivated samples.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.