Storage

For short term storage Vascular Cell Adhesion Molecule-1 Fragment (Western Blot Control) Protein may be kep at +4 deg. C. For long term storage keep the aliquoted vials frozen at -20 degrees Celsius.

Tips

Due to handling and transportation, small amounts of the Vascular Cell Adhesion Molecule-1 Fragment (Western Blot Control) Protein may get caught on the walls or seal of the vials. Prior to use briefly rund the vial(s) through a centrifuge to collect all of the content on the bottom. Avoid freezing and thawing the vials numerous times as such cycles may denaturate the peptide chains and influence the item's performance.

Tissue

cell, control, vascular

Description

cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Vascular Adhesion Molecule-1 ( Blot Control) Protein.For cells, cell lines and tissues in culture till half confluency.Isotype or positive controls by peptides, antibodies and deactivated samples.Whole adhesion and interacting molecules are  present in lysates used as reference for ELISA quantification of these molecules and their subunits.

Test

Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.