Host/Source

Mouse

Clone Name

VI-AP

Product Category

NA

Species Reactivity

NA

Application

Direct Immunofluorescence, Indirect Immunofluorescence, Flow Cytometry

Immunogen

NA

Purification Method

NA

Shipping Conditions

NA

Storage conditions

IgG1 Negative Control, conjugated to FITC, Negative Control MUbio monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8ÁC (DO NOT FREEZE!) and protected from prolonged exposure to light. Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended. _x000B_

Datasheet link

NA

Tissue

control

Description

Isotype or positive controls by peptides, antibodies and deactivated samples.Negative control peptides or isotypes available of the different species.

Properties

This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.

Conjugation

Anti-FITC Antibody

Additional isotype

IgG1