Host/Source

Goat

Clone Name

NA

Product Category

Veterinary

Species Reactivity

Caprine

Application

ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence

Immunogen

NA

Purification Method

NA

Shipping Conditions

NA

Storage conditions

Goat IgG, conjugated with FITC, Isotype control lized at +4ÁC--at least 10 years. Reconstituted at or below -20ÁC--3-5 years reconstituted at +4ÁC--7 days

Datasheet link

NA

Description

Isotype or positive controls by peptides, antibodies and deactivated samples.

Properties

This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

Conjugation

Anti-FITC Antibody

Latin name

Capra aegagrus hircus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG

Group

isotypes