Stock availability
Available
Category
Isotype Control
Antibody type
N/A
Antibody host
N/A
Antibody conjugate
Biotin
Technical datasheet
Contact Gentaur to request the datasheet or ask our specialists for more information.
Notes
The Mouse IgG Fab-Biotin conjugate (isotype control) is manufactured for Research Use Only or for diagnostics purposes.
Tissue
control
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent,Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
Conjugation
Biotinylated
Description
Isotype or positive controls by peptides, antibodies and deactivated samples.
Additional isotype
Fab fragment,IgG
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Group
isotypes
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus