Human IgE-Biotin (myeloma, kappa), purified (isotype control, >95%, low endotoxin, azide free)

General information
 

Name:
Human IgE-Biotin (myeloma, kappa), purified (isotype control, >95%, low endotoxin, azide free)
Size:
100 ul
Catalog no:
20007-3-LE-BTN
Price:
392 EUR
 

Additional extra details
 

  • Stock availability

    Available

    Category

    Isotype Control

    Antibody type

    N/A

  • Antibody host

    N/A

    Antibody conjugate

    Biotin

    Technical datasheet

    Contact Gentaur to request the datasheet or ask our specialists for more information.

  • Notes

    The Human IgE-Biotin (myeloma, kappa), purified (isotype control, >95%, low endotoxin, azide free) is manufactured for Research Use Only or for diagnostics purposes.

    Tissue

    control

    Description

    Sodium azide NaN3 is often used to prevent antibody degradation in 0,02% W/V concentrations. adi supplies azide free antibodies on request.Recently adi made this IgE- (myeloma, kappa) purified (isotype control, >95% endotoxin, ) available without azide. It will stick less and is more suitable for ELISA or in vivo studies. However you need to store the @VV@ frozen and ship on dry ice.Isotype or positive controls by peptides, antibodies and deactivated samples.

  • Properties

    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.Immunoglobulin E (IgE) kappa or Fc specific antibody is a kind of antibody (or immunoglobulin (Ig) "isotype") that has only been found in mammals. IgE is synthesized by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing 4 Ig-like constant domains (Cε1-Cε4). IgEs play a role in allergy and response to parasite infection. High levels of IgEs caused by parasites can lower the allergic reaction of patients to allergens common present in the human environment. As such the parasite history of an allergic patient needs to be taken in consideration as a positive factor.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

    Conjugation

    Biotinylated

    Additional isotype

    IgE

  • Group

    isotypes