If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
Isotype or positive controls by peptides, antibodies and deactivated samples.Positive controls are the same as the target vector or antibody or protein and can be spiked to the sample before the analysis starts.