IgG1, Murine Isotype Control (FITC)

General information
 

Name:
IgG1, Murine Isotype Control (FITC)
Size:
100 Tests
Catalog no:
MBS655558
Price:
442 EUR
 

Additional extra details
 

  • Other size

    please contact us to order other different size

    Tissue

    control

    Description

    Isotype or positive controls by peptides, antibodies and deactivated samples.

  • Properties

    This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

    Conjugation

    Anti-FITC Antibody

    Group

    isotypes