IgG2b Isotype Control Fc fusion protein (biotin)

General information
 

Name:
IgG2b Isotype Control Fc fusion protein (biotin)
Size:
100 ug
Catalog no:
35R-0021
Price:
315 EUR
 

Additional extra details
 

  • Research Area

    Immunology

    Product Subtype

    Isotype Controls

    Product Type

    Biological Reagents

  • Concentration

    500 ug/ml

    Form and Buffer

    aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.

    Storage

    Store at 2-8 deg C. Do not freeze. Protect from light.

  • Shipping Info

    Blue Ice

    Applications

    FC, ELISA

    Tissue

    control

  • Properties

    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

    Conjugation

    Biotinylated

    Description

    Isotype or positive controls by peptides, antibodies and deactivated samples.Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.

  • Group

    isotypes